REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit monoclonal anti-camelid HRP	GenScript	A01681
Rabbit polyclonal anti-camelid HRP	GenScript	A02016
Mouse monoclonal anti-α-tubulin	ThermoFisher	PA5-22060
Mouse monoclonal anti-GFP	Roche	1814460001
Mouse monoclonal anti β-actin	Sigma-Aldrich	A5316
Sheep polyclonal anti NEDP1	Bailly et al., 2019	N/A
Rabbit monoclonal anti NEDD8	Epitomics	1571-1
Rabbit monoclonal anti TRIM28	Cell Signaling Technology	4124
Chemicals, Peptides, and Recombinant Proteins
Doxycycline Hydrochloride	Sigma-Aldrich	D3447
Proteasome inhibitor MG132	Sigma-Aldrich	C2211
Proteasome inhibitor Bortezomib	Selleckckem	PS0341
Autophagy inhibitor Bafilomycin A1	ENZO	BML-CM110-0100
the KOD Hot Start DNA Polymerase	Merck Millipore	71086
FastDigest Restriction enzymes	Thermo Fisher/Fermentas	N/A
Rapid DNA Ligation Kit	Roche	11635379001
DAPI Stain	Thermofisher	62248
HCS cellmask red stain	Thermofisher	H32712
6His-GFP-Sumo1	This study	N/A
6His-NEDP1	This study	N/A
RNF4 2xRING	This study	N/A
Ubiquitin	This study	N/A
UbcH5a	This study	N/A
MBP-NEDD8-Ub	This study	N/A
GNb-1xRING	This study	N/A
GNb-2xRING	This study	N/A
GNb-1xmtRING	This study	N/A
GNb-2xmtRING	This study	N/A
NNb2-1xRING	This study	N/A
NNb2-2xRING	This study	N/A
NNb7-1xRING	This study	N/A
NNb7-2xRING	This study	N/A
NNb9-1xRING	This study	N/A
NNb9-2xRING	This study	N/A
Critical Commercial Assays
Lipofectamine 3000	Life Technologies	L3000015
Lipofectamine RNAiMAX	Life Technologies	13778150
Neon Transfection System 100 μL Kit	Life Technologies	MPK10025/MPK10096
Neon Transfection System 10 μL Kit	Life Technologies	MPK1096/ MPK1025
mMESSAGE mMACHINE T7 ULTRA Transcription Kit	Thermo Fisher	AM1345
MEGAclear Transcription Clean-Up Kit	Thermo Fisher	AM1908
QIAquick MinElute Gel Extraction Kit	QIAGEN	28604
Pierce BCA Protein Assay Kit	Thermo Fisher	23225
E.Z.N.A Total RNA Kit	VWR	R6834
First Strand cDNA Synthesis Kit	Thermo Fisher	K1612
PerfeCTa® SYBR® Green RT-PCR kit	Quanta Biosciences	95054-500
Deposited Data
Study original experimental raw data deposited to Mendeley.	https://dx.doi.org/	https://doi.org/10.17632/ygvn5hmv78.1
Mass spectrometry data deposited to the ProteomeXchange Consortium	https://www.ebi.ac.uk/pride	PXD018113
Experimental Models: Cell Lines
HeLa	ATCC	CCL-2
HEK293	ATCC	CRL-1573
HeLa Flp-in/T Rex	Thermo Fisher	R71407
HeLa Flp-in/T Rex GNb-1xRING	This study	N/A
HeLa Flp-in/T Rex GNb-2xRING	This study	N/A
HeLa Flp-in/T Rex GNb-1xRING EYFP-PARG	This study	N/A
HeLa Flp-in/T Rex GNb-2xRING EYFP-PMLIII	This study	N/A
HeLa Flp-in/T Rex NNb2-1xRING	This study	N/A
HeLa Flp-in/T Rex NNb2-1xmtRING	This study	N/A
HeLa Flp-in/T Rex NNb9-2xRING	This study	N/A
HeLa Flp-in/T Rex NNb9-2xmtRING	This study	N/A
HEK293 EYFP-SP100	This study	N/A
MCF7 GFP-tubulin	Gift from Prof. Jason Swedlow	N/A
Oligonucleotides
DNA primers: see Table S3		N/A
Recombinant DNA
pOG44	Invitrogen	V600520
pCDNA5 FRT TO	Invitrogen	V652020
pCDNA5 FRT TO GNb-1xRING	This study	N/A
pCDNA5 FRT TO GNb-2xRING	This study	N/A
pCDNA5 FRT TO NNb2-1xRING	This study	N/A
pCDNA5 FRT TO NNb2-1xmtRING	This study	N/A
pCDNA5 FRT TO NNb9-2xRING	This study	N/A
pCDNA5 FRT TO NNb9-2xmtRING	This study	N/A
pCDNA5 FRT TO NNb7-1xRING	This study	N/A
pCDNA5 FRT TO NNb7-2xRING	This study	N/A
pLou3 GNb-1xRING	This study	N/A
pLou3 GNb-1xmtRING	This study	N/A
pLou3 GNb-2xRING	This study	N/A
pLou3 GNb-2xmtRING	This study	N/A
pLou3 NNb2-1xRING	This study	N/A
pLou3 NNb2-2xRING	This study	N/A
pLou3 NNb7-1xRING	This study	N/A
pLou3 NNb7-2xRING	This study	N/A
pLou3 NNb9-1xRING	This study	N/A
pLou3 NNb9-2xRING	This study	N/A
pEFIRES-P-EYFP-C1	This study	N/A
pEFIRES-P-EYFP-C1 PARG	This study	N/A
pEFIRES-P-EYFP-C1 RNF146	Medical Research Council Protein Phosphorylation and Ubquitilation Unit Reagents and services https://mrcppureagents.dundee.ac.uk/reagents-cdna-clones/overview	DU20596
pEFIRES-P-EYFP-C1 PEX10	Medical Research Council Protein Phosphorylation and Ubquitilation Unit Reagents and services https://mrcppureagents.dundee.ac.uk/reagents-cdna-clones/overview	DU20598
pCMV EYFP-IRESpuro PMLIII	This study	N/A
Software and Algorithms
IN Cell Developer Toolbox	GE Healthcare	version 1.91 build 2206
MaxQuant software	Cox and Mann, 2008	version 1.6.1.0
Skyline Targeted Mass Spec Environment	https://skyline.ms/project/home/software/Skyline/begin.view	Version 19.1.0.193
ImageJ	NIH, https://imagej.nih.gov/ij/	Version 1.49K