Figure 3.

Specific MAPK pathways regulate the IL-10-inhibition of inflammatory mediators in chlamydial macrophages and the induction of SOCS expression. Macrophages (10⁶/mL) were pre-incubated for 1 h with MAPK pathway specific-inhibitors (each at 20 μM) for p38 (SB203580), JNK (SP600125) and MEK1/2 (UO126) followed by stimulation for 24 h with rMOMP (10 μg/mL) in the presence and absence of IL-10 (10 ng/mL). IL-6 (A) and CCL5 (B) were measured by specific ELISAs. TaqMan qRT-PCR was employed to quantify SOCS1 and SOCS3 mRNA gene transcripts (C). Results are presented as fold increase over the control. An asterisk indicates significant differences (P <0.05), and P values were calculated as described in Figure 1 legend. Each bar represents the mean ± SD of samples run in triplicates. Each experiment was repeated 3 times.