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Load-dependency of single-beat Ees estimation. Comparison of end-systolic elastance (Ees) obtained as the slope of end-systolic pressure-volume relation (red dashed line, ESPVR) and single-beat estimation (solid black lines, Eessb1 and Eessb2). When V0 =0 was assumed, the evaluation of Ees by the single-beat method (Eessb = end-systolic pressure or Pes/end-systolic volume) at different levels of afterload results in different slopes, while the actual Ees remains unchanged.
