As the threshold becomes more stringent, sequences break up from the main cluster. Nodes have been colored according to sequence length in amino acids (scale at the bottom). A: At threshold values below 50, proteins sequence are divided in two main clusters. B: At a threshold value of 75, the first group of sequences becomes independent from the main clusters. This group (red circle) contains proteins from the DesA family. C: At a threshold value of 100, there is a clear separation into multiple groups. Proteins from the DesA family remain clustered (red circle), and proteins from the DesB family are separated into their own cluster (cyan circle). D: At threshold values above 150, the majority of sequences break up into disconnected groups.