Skip to main content
. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 20;199:105567. doi: 10.1016/j.jsbmb.2019.105567

Figure 4. Side-chain cleavage of endogenous and glucocorticoid drugs by whole cells of gut bacterium Clostridium scindens ATCC 35704 and urinary tract isolate Propionimicrobium lymphophilum ACS-093-V-SCH5.

Figure 4.

A: Steroid-17,20-desmolase reaction showing endogenous (highlighted in gray) and exogenous (highlighted in blue) compounds as substrates. Anticipated product (loss of C2O2H4) of side chain cleavage is 60.02 m/z smaller than substrate. B: LC/MS in single ion monitoring (SIM) mode of extracts from C. scindens incubated with 50 μM substrate. C: LC/MS in single ion monitoring (SIM) mode of extracts from P. lymphophilum incubated with 50 μM substrate. Chromatographs representative of three biological replicates.