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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Steroid Biochem Mol Biol. 2019 Dec 20;199:105567. doi: 10.1016/j.jsbmb.2019.105567

Figure 5. SDS-PAGE of purified recombinant DesA and DesB from Clostridium scindens ATCC 35704 and NADPH-dependent 17β-HSDH from Cochliobolus lunatus.

Figure 5.

Affinity purified recombinant steroid-17,20-desmolase (rDesA and rDesB) co-expressed in pETDuet (Lane DesAB), affinity purified recombinant 17β-hydroxysteroid dehydrogenase expressed in pET21 (Lane 17β-HSDH), and molecular mass marker (Lane M).