TABLE 1.
Target gene | Primer sequence (5′-3′) | Product size | Protein encoded | Serotype specificity |
LMxysn_1095 | F: AATACTTGGACAGACGGAACGC | 602 bp | Galactosyltransferase | Serotypes 4h, 4b, 4d, and 4e |
R: TCATCTGGCTCTTTTAGAACCG | ||||
lmo1083 | F: CACAAATGGTCTTGACGGGG | 390 bp | L-rhamnosyl transferase | Serotypes 1/2, 3, and 7 and Listeria seeligery (1/2b) |
R: TTTGCGCGTGATTTTAGTGG | ||||
smcL | F: CACAGACCATTGTGGTGACTTG | 889 bp | Sphingomyelinase C | Serotype 4h and L. ivanovii |
R: CGGTGCTTTCATTTTTTTACTC |
F, forward primer; R, reverse primer.