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. 2020 Jun 26;11:1424. doi: 10.3389/fmicb.2020.01424

TABLE 2.

Community parameters obtained from Illumina sequencing data of the V6-V8 region of 16S rRNA genes, from rhizospheric samples associated with E. platyacanthus (Ep) and N. polylopha (Np), cultivated (C) and wild (W). Presented parameters and their standard deviation values are treatment estimators of species richness (Observed ASV, Chao 1), Shannon’s (H’) and Simpson’s (D) diversity indices, and Pielou’s (J) evenness index. Calculations were done after rarefying the respective classified reads (see Supplementary Table S1), to 5,213 sequence reads, corresponding to the smallest number of classified reads in sample NpW-2.

Sample group Number of observed ASV Chao1 Shannon H’ Simpson D Evenness J Good’s coverage (%)
EpC 758 ± 51a 781 ± 61a 6.31 ± 0.08a 0.9975 ± 0.0003a 0.9471 ± 0.0025a 99.99
EpW 649 ± 92a 666 ± 91a 6.08 ± 0.15a 0.9963 ± 0.0006a 0.9358± 0.0024ab 99.99
NpC 528 ± 132ab 537 ± 140ab 5.95 ± 0.21a 0.9963 ± 0.0005a 0.9504 ± 0.0056a 99.99
NpW 310 ± 110b 311 ± 110b 5.23 ± 0.32b 0.9900 ± 0.0020b 0.9193 ± 0.0200b 99.99

Values followed by different letter are statistically significant as tested by one-way ANOVA and Tukey HSD analysis (P-value < 0.05).