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. 2020 Jul 3;11:269. doi: 10.1186/s13287-020-01785-6

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Downregulation of HDAC9 rescued lineage differentiation imbalance and ameliorated senescence in aged BMMSCs. a Alizarin Red staining was performed, and osteogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. b Oil Red O staining was performed, and adipogenesis-related proteins were detected by western blotting in aged BMMSCs transfected with HDAC9 siRNA. c Alizarin Red staining was performed, and osteogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. d Oil Red O staining was performed, and adipogenic-related proteins were detected by western blotting in young BMMSCs and young BMMSCs transfected with HDAC9 siRNA. e, f Expressions of the senescence-related proteins p53 and p-p53 in BMMSCs cultured in vitro from aged mice (e) and young mice (f) were examined by western blotting. The data are presented as the means ± SD of each independent experiment performed in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001, one-way analysis of variance (ANOVA)