FIGURE 1.

SGIV infection inhibited autophagy in GS cells. (A) SGIV infection altered protein levels of LC3, Beclin1, and p-mTOR as determined by Western blot analysis. β-tubulin was used as the internal control. Band intensity was calculated using Quantity-one software, and the ratio of target protein/β-tubulin was shown below the blot. (B) The expression of LC3 was detected by Western blot analysis in infected or non-infected SGIV cells after Rap pre-treatment. Band intensity was calculated using Quantity-one software, and the ratio of LC3-II/β-tubulin was shown below the blot. (C) Flow cytometry-based profiling of autophagy in infected (12 h p.i.) or non-infected SGIV cells after Rap pre-treatment. Cyto-ID dye was used to stain cells, and 1 × 10⁴ cells were collected for further positive analysis. The data were presented as the means from three independent experiment.