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. 2020 Jun 26;11:1446. doi: 10.3389/fmicb.2020.01446

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Copyright © 2020 Li, Wang, Liu, Yu, Huang, Huang, Wei and Qin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SGIV-VP48, VP122, and VP132 interacted with Atg5 and their ectopic expression decreased the level of LC3-II. (A) HA-Atg5 interacted with GFP-VP122. Whole cell lysates (WCL) transfected with GFP-VP122 and HA-Atg5 or HA-LC3 were subjected to immunoprecipitation (IP) and immunoblotting (IB) with indicated antibodies. (B) HA-Atg5 interacted with GFP-VP132. WCLs of cells transfected with GFP-VP132 and HA-Atg5 or HA-LC3 were used for IP and IB with indicated antibodies. (C) HA-Atg5 interacted with GFP-MAVS, GFP-CARD, and GFP-VP48. WCLs of cells transfected with HA-Atg5 and GFP-MAVS, GFP-CARD, or GFP-VP48 were used for IP and IB with indicated antibodies. (D) VP48, VP122, and VP132 decreased the level of LC3-II. Cells transfected with HA-VP48, HA-VP122, or HA-VP132 were collected for Western blot analysis. β-tubulin was used as the internal reference. Band intensity was calculated using Quantity-one software, and ratios of LC3-II/β-tubulin were assessed. The data were presented as the means from three independent experiment.