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. 2020 Jun 26;11:1446. doi: 10.3389/fmicb.2020.01446

FIGURE 4.

FIGURE 4

Rap decreased SGIV replication, whereas WM promoted SGIV replication. GS cells were pretreated with Rap or WM or the same dosage of DMSO for 2 h prior to SGIV infection, then the infected cells were collected and analyzed by qPCR and immunofluorescence assay (IFA). (A,D) The LC3 expression in Rap or WM pretreated cells. (B,E) The relative expression of MCP, ICP18, VP19, and LITAF in SGIV infected cells. The β-actin gene was used as the internal reference for qPCR. (C,F) The infected cells were analyzed at 12 h p.i. by IFA using anti-MCP antibody (green). Nuclei were stained by DAPI (blue). The viral infectivity of control (DMSO treated) cells was set as 1. The data were presented as the means from three independent experiment, *p < 0.05, **p < 0.01.