Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 26;11:1446. doi: 10.3389/fmicb.2020.01446

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Li, Wang, Liu, Yu, Huang, Huang, Wei and Qin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

LC3 overexpression decreased SGIV replication, whereas LC3 knockdown promoted SGIV replication. GS cells were transfected with HA-LC3 or the vector, siLC3 or the negative control (NC), and then infected with SGIV. Viral replication was analyzed by qPCR and IFA. (A,D) The LC3 expression in transfected cells. (B,E) The relative expression of MCP, ICP18, VP19, and LITAF in SGIV infected cells. The β-actin gene was used as the internal reference for qPCR. (C,F) The infected cells were analyzed at 12 h p.i. by IFA using anti-MCP antibody (green). Nuclei were stained by DAPI (blue). The viral infectivity of control cells was set as 1. The data were presented as the means from three independent experiment, *p < 0.05, **p < 0.01.