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. 2020 Jun 26;11:950. doi: 10.3389/fpls.2020.00950

Figure 1.

Figure 1

Sequence analysis of AaABCG40. (A) Phylogenetic analysis of AaABCG40 from A. annua and some known PDR transporters from Arabidopsis, A. annua AaPDR3, N. plumbaginifolia NpPDR1, N. tabacum NtPDR1, and S. polyrrhiza SpTUR2. The tree presented here is a neighbor-joining tree based on amino acid sequence alignment. (B) The structure of AaABCG40 was predicted by scanning the deduced amino acid sequence. NBD and TMD indicate the predicted location of NBDs and TMDs, respectively. (C) Multiple alignment of the conserved domain of known PDR transporters involved in terpene transport has the high conservation in plants. The Walker A, Walker B and ABC signature motifs are shown with shading. The identical amino acid residues in are marked by asterisks. (D) Relative expression of AaABCG40 in root, stem, old leaf, young leaf, bud, flower, shoot and trichome. (E) Relative expression of AaABCG40 in leaves of different developmental ages of A. annua. ACTIN was used as internal control. The error bars represent the means ± SD (standard deviation) from three biological replicates.