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. 2020 Jun 26;11:979. doi: 10.3389/fphar.2020.00979

Figure 1.

Figure 1

Ligustilide (LIG) promotes viability of hippocampal neurons induced by oxygen-glucose deprivation/reperfusion (OGD/R). (A) Chemical structure of LIG. B.Primary culture and identification of hippocampal neurons. (B-a) The seventh day of primary culture of hippocampal neuron by light microscope. (B-b) Hippocampal neuron were identified by MAP-2 immunostaining. (C) Hippocampal neurons were treated with LIG at various concentrations(0.5–128 μM) and the cell viability was measured by the MTT assay. (D) Effect of ligustilide on the release of LDH from hippocampal neurons damaged by OGD/R. (E) Cell viability was measured by the MTT assay after OGD/R and normal conditions. All data are expressed as means ± SD (n = 8). ## P < 0.01 vs. control group; **P < 0.01 vs. group treated with OGD/R.