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. 2020 Jun 26;11:979. doi: 10.3389/fphar.2020.00979

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Copyright © 2020 Wu, Mao, Liu, Huang and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of ligustilide on oxygen-glucose deprivation/reperfusion (OGD/R)-induced apoptosis of hippocampal neurons. (A) The percentage of hippocampal neurons apoptosis was analyzed by staining cells with Hoechst 8 (blue represents viable cells, ×200). (B, C) Apoptotic cells rate were detected by flow cytometry. (a) Control group; (b) OGD/R; (c) 1 μM LIG+OGD/R; (d) 2μM LIG+OGD/R; (e) 4 μM LIG+OGD/R. (D) Western blot image and the expression analysis of Cyt C in mitochondrial and cytosolic fractions. (E) Western blot image and the expression analysis of Bcl-2 and Bax. Data shown are the results of three different experiments. ^## P < 0.01 vs. control group; *P < 0.01, **P < 0.01 vs. group treated with OGD/R.