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. 2020 Jun 26;10:975. doi: 10.3389/fonc.2020.00975

Figure 3.

Figure 3

The GAP domain is required for both glioma and neuroblastoma cell migration. (A) The construct scheme of the domain-deleted plasmids of Porf-2. Verification of domain-deleted plasmids via western blotting with the HA antibody. The same blot was probed with GAPDH antibody as a loading control. (B) Representative images of the wound healing assay of N-2a cells at different time points in different groups. Quantification of the wound area at indicated time points in different groups. Scale bar, 200 μm. (C) Representative images of the wound healing assay of U87 cells at different time points in different groups. Scale bar, 200 μm. (D) Representative images of the migrated N-2a cells in different groups. Quantification of the migrated cells in each group. Scale bar, 200 μm. (E) Representative images of the migrated U87 cells in different groups. Quantification of the migrated cells in each group. Scale bar, 200 μm. Error bars represent ± SEM; ns indicates no significant difference (P > 0.05). **P < 0.01, ***P < 0.001.