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. 2020 Jun 26;10:975. doi: 10.3389/fonc.2020.00975

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Copyright © 2020 Li, Huang, Liu, Yang, Wang, Luo, Liang, Yuan, Zhen and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Porf-2 inhibits tumor cell migration through MMPs. (A) CCK-8 assays were performed to analyze the proliferation of N-2a cells in different groups. (B) CCK-8 assays were performed to assess the proliferation ability of U87 cells in different groups. Error bars represent ± SEM; ^**P < 0.01, ^***P < 0.001 vs. the control group. (C) Real-time PCR analysis was performed to explore the relative mRNA expression of the potential target genes after Porf-2 overexpression in N-2a. The relative mRNA level was normalized to the control. The red line indicates the normalized control group. (D) Real-time PCR was performed to verify the downexpression of MMPs after Porf-2 overexpression in U87 cells. The relative mRNA level was normalized to the control. The red line indicates the normalized control group. Error bars represent ± SEM; ^**P < 0.01, ^***P < 0.001 vs. the control group.