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lso− mutants show poor survival in stationary phase. (A) Promastigotes were inoculated in M199 medium at 1 × 105/ml, and cell densities were determined daily. The arrow marks the onset of stationary phase. (B and C) Percentage of round cells (B) and dead cells (C) were measured during days 1 to 4 in stationary phase. Error bars represent standard deviations from 3 experiments (**, P < 0.01; ***, P < 0.001).
