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. 2020 Jul 1;5(4):e00380-20. doi: 10.1128/mSphere.00380-20

FIG 8.

FIG 8

lso− mutants have fewer short side chain sugar residues on their LPG. (A) Whole-cell lysates were processed for Western blotting with MAb CA7AE or anti-α-tubulin antibody. Log-phase (left three lanes) and day 3 stationary-phase (right three lanes) promastigotes of LV39 WT, lso−, and lso−/+LSO strains were analyzed along with L. donovani strains 1S2D and LV82 (middle two lanes). PPG, proteophosphoglycan. (B) Immunoblotting of purified LPG (5 μg per lane) from L. major strains probed with MAb WIC79.3. (C) FACE analysis of dephosphorylated LPG repeat units from L. major (lanes 2 to 5). Lane 1, malto-oligomer ladder represented by 1 to 7 glucose residues (G1 to G7). (D) Monosaccharide profile of GIPLs. Lane 1, glucose standard; lane 2, type I GIPL from L. donovani 1S2D containing mostly mannose residues and low levels of galactose; lane 3, repeat units of L. major LV39 WT (type II GIPL); lane 4, repeat units of L. major lso−; and lane 5, repeat units of L. major lso−/+LSO. Man, mannose; Glc, glucose; Gal, galactose. Experiments were performed twice, and results from one representative set are shown.