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lso− mutants show attenuated virulence in mice. BALB/c mice were infected in the footpads with metacyclics (A and B) or amastigotes (C and D). Footpad lesions were measured weekly (A and C), and parasite numbers were determined by the limiting dilution assay (B and D). Error bars represent standard deviations (*, P < 0.05; **, P < 0.01).
