TABLE 2.
LSO is required for the maintenance of intracellular pHa
| Cell type | Intracellular pH at medium pH of: |
|||
|---|---|---|---|---|
| 7.4 |
5.0 |
|||
| Mean | SD | Mean | SD | |
| WT | 7.76 | 0.265 | 7.90*** | 0.122 |
| lso− | 7.48 | 0.265 | 7.05*** | 0.031 |
| lso−/+LSO | 7.83 | 0.124 | 7.78 | 0.130 |
a
Promastigotes were cultivated in the regular medium (pH 7.4) or acidic medium (pH 5.0) for 3 days, and intracellular pH values were measured after labeling with 10 μM of BCECF-AM for 30 min. Mean values and standard deviations (SDs) were calculated from 3 experiments (***, P < 0.001).