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. 2020 May 15;183(3):854–868. doi: 10.1104/pp.20.00156

Figure 1.

Figure 1.

Expression patterns of SlMYB72 gene and subcellular localization analysis of SlMYB72 protein in tomato. A, RT-qPCR analysis of SlMYB72 expression level. Bars from left to right are as follows: Rt, root; St, stem; Lf, leaf; Fl, flower; Img, early immature green fruit; Img2, late immature green fruit; Mg, mature green fruit; Bf, breaker fruit; Of, orange fruit; Rf, red fruit. The data represent means ± sd of four biological replicates. B, Expression pattern of SlMYB72 revealed by the expression of the GUS reporter gene driven by the SlMYB72 promoter. Bars = 1 mm. C, Subcellular localization analysis of SlMYB72 protein. The SlMYB72-GFP fusion protein was transiently expressed in Nicotiana benthamiana leaves. Nuclei were identified by 4′,6-diamidino-2-phenylindole (DAPI) staining. Bars = 50 μm. D, Transcriptional activation activity of SlMYB72 protein. pGBKT7-SlMYB72 fusion vector, negative control, and positive control were transformed into Y2HGold yeast cells. The yeast cells were cultivated on SD-Trp/His/Ade.