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. 2020 May 15;183(3):854–868. doi: 10.1104/pp.20.00156

Figure 2.

Figure 2.

Generation of RNAi-SlMYB72 plants and analysis of chlorophyll accumulation and chloroplast development in RNAi-SlMYB72 plants. A, RT-qPCR analysis of the expression of SlMYB72 in transgenic lines. The data represent means ± sd of four biological replicates. The relative expression levels of genes were compared between the RNAi-SlMYB72 (RNAi) and wild-type (WT) plants. Different lowercase letters indicate significant differences. B, Fruit phenotypes. Bars = 0.5 cm. C, Chlorophyll a contents in mesocarps, exocarps, and pericarps of wild-type and RNAi-SlMYB72 fruits. D, Chlorophyll b contents in mesocarps, exocarps, and pericarps of wild-type and RNAi-SlMYB72 fruits. FW, Fresh weight. E, TEM observation of chloroplasts in mesocarps of wild-type and RNAi-SlMYB72 fruits. Bars = 2 μm (left) and 500 nm ( right). F, Analysis of chloroplast size. G, Number of chloroplasts per cell. The data represent means ± sd of three biological replicates in C, D, F, and G. Asterisks indicate significant differences between the transgenic and wild-type plants (*P < 0.05 and **P < 0.01), as determined by Student’s t test.