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. 2020 May 15;183(3):854–868. doi: 10.1104/pp.20.00156

Figure 7.

Figure 7.

SlMYB72 targets the genes involved in the biosynthesis of carotenoids and flavonoids. A, Expression levels of PSY, ZISO, and LCYB genes in pericarps of red fruits. RNAi, RNAi-SlMYB72 lines; WT, wild-type plants. The data represent means ± sd of four biological replicates. The relative expression levels of genes were compared between the RNAi-SlMYB72 and wild-type plants. B to D, EMSA showing the binding of SlMYB72 to the promoters of PSY, ZISO, and LCYB genes, respectively. Biotin-labeled DNA probes from native promoter or mutants were incubated with GST-SlMYB72 protein, and the DNA-protein complexes were separated on 6% native polyacrylamide gels. E, ChIP-qPCR assay for the direct binding of SlMYB72 to PSY, ZISO, and LCYB genes. Values are percentages of DNA fragments that coimmunoprecipitated with anti-FLAG antibodies or nonspecific antibodies (anti-IgG) relative to the input DNA. F, Expression levels of 4CL, CHS1, and CHS2 genes in pericarps of red fruit. G to I, EMSA showing the binding of SlMYB72 to the promoters of 4CL, CHS1, and CHS2 genes, respectively. J, ChIP-qPCR assay for the direct binding of SlMYB72 to the 4CL, CHS1, and CHS2 genes. The data represent means ± sd of four biological replicates in A, E, F, and J. Asterisks indicate significant differences between transgenic and wild-type plants (**P < 0.01), as determined by Student’s t test.