Table 3. Oxidative modifications identified by LC-MS/MS on the recombinant sPPase protein p26b after H2O2 treatment.
Oxidative modifications identified on the recombinant proteinp26b and its phosphomimic mutant p26b(3′E) and phosphonull mutant p26b(3′A) without and after H2O2 treatment. Bold text indicates irreversible oxPTMs. I, Irreversible; R, reversible. Details of oxidative modifications relating to these experimental data are in Supplemental Tables S8, S9, S10, and S11 and Supplemental Figure S2
| Residue | Untreated | H2O2-Treated | ||
|---|---|---|---|---|
| p26b | p26b(3′E) | p26b(3′A) | ||
| M1 | Met sulfoxide (R) | Met sulfoxide (R) | Met sulfoxide (R) Met sulfone (I) | Met sulfoxide (R) |
| M150 | – | Met sulfoxide (R) | Met sulfoxide (R) | Met sulfoxide (R) |
| M152 | – | – | Met sufoxide (R) | – |
| M223 | Met sulfoxide (R) | Met sulfoxide (R) Met sulfone (I) | Met sulfoxide (R) Met sulfone (I) | Met sulfoxide (R) Met sulfone (I) |