Figure 2.
Effects of mevinolin and fosmidomycin on carotenoid synthesis in C. zofingiensis. A and B, TC and astaxanthin as affected by different concentrations (micromolar) of mevinolin (A) and fosmidomycin (B). Cells were cultured under ND conditions for 48 h. Asterisks indicate significant differences (Student’s t test, *P < 0.05) compared with the control without inhibitors (0 μm). C, Transcriptional expression of DXS, DXR, and HDR genes with or without 100 μm fosmidomycin (inhibitor). The gene expression level was normalized to the internal control β-actin gene. Asterisks indicate significant differences (Student’s t test, *P < 0.05) in gene expression between samples treated with or without fosmidomycin. Data represent means ± sd (n = 3).