Figure 7.

Comparative transcriptomic analysis of ND-induced C. zofingiensis cells with and without cerulenin. A, Venn diagram of shared DEGs between 6 and 12 h of ND. The values in red indicate the number of up-regulated DEGs, whereas the values in blue designate the number of down-regulated DEGs in the presence of cerulenin compared with no cerulenin. B, Functional distribution of DEGs in up- and down-regulated categories, as follows: 1, function unknown; 2, cell cycle; 3, stress and cell death; 4, transport; 5, energy; 6, cell structure and component; 7, protein synthesis, modification, folding; and turnover; 8, DNA, RNA, and gene expression; 9, signaling; 10, metabolism; 11, photosynthesis. C, Transcriptional regulation of certain carbon metabolism pathways. Genes (italic) that are differentially expressed are shown in red (up-regulated) and blue (down-regulated). Compounds (roman) are shown in different colors: red, increased; blue, decreased; black, not determined or nonchanged. The pathways boxed in black indicate nonregulation. Values in parentheses designate fold changes of compound variation (48 h). The heat map shows log₂ (fold change) values of transcripts. ACS, Acetyl-CoA synthetase; AK, acetate kinase; FA, fatty acid; MLs, membrane lipids; OPP, oxidative pentose phosphate pathway; PAT, phosphate acetyltransferase; PDC, pyruvate decarboxylase; TCA, tricarboxylic acid cycle. See Supplemental Data Set S2 for more details of the RNA-seq data.