Figure 1.

Expression of OsWAKL21.2 is enhanced in rice leaves after treatment with either LipA or Xoo. A, Venn diagram indicating the number of genes that are differentially expressed after 2 and 12 h of LipA treatment. B, Three splice variants of OsWAKL21 as shown in the Rice-MSU database. C, RT-qPCR analysis of the expression of all three splice variants of OsWAKL21 after 2 and 12 h of LipA treatment and after 24 h of Xoo treatment in rice leaves. Asterisks represent significant differences in FC using Student’s t test with P < 0.05. n.s. indicates no significant difference in relative expression. D, RT-qPCR analysis of the expression of OsWAKL21.2 in rice leaves after 24 h of treatment with Xoo, LipA mutant of Xoo (Xoo LipA-), or LipA-complementing clone of Xoo (Xoo LipA-CC). Lowercase letters (a and b) above the bars indicate significant differences in FC using Student’s t test with P < 0.05. In C and D, 12- to 14-d-old leaves were infiltrated with either LipA (0.5 mg mL⁻¹) or Xoo (OD of 1). Each bar represents the average value, and error bars denote se of at least three independent experiments. Relative expression was calculated in leaves treated with LipA or Xoo with respect to leaves treated with buffer. OsACTIN1 was used as an internal control for RT-qPCR. The relative FC was calculated by using the 2^−∆∆Ct method.