Table 3. Genes encoding RLKs, small GTPases, and HXTs were differentially expressed in pSTK-amiRNACWIN24 during ovule initiation compared with the wild type.
RPKM values were derived from four biological replicates of floral buds at stages 8 to 10, when ovules initiate, with each replicate comprised of 10 to 15 buds from one plant.
| Functional Category | Gene Identifier | Average RPKM | Log2 FC | Annotation | |
|---|---|---|---|---|---|
| Wild Type | Transgenic | ||||
| Signaling | |||||
| RLKs | AT5G59670 | 4.06 | 8.89 | 1.05 | LRR protein kinase, putative |
| AT2G07040 | 1.79 | 0.08 | −4.51 | ATPRK2A, PRK2A | PRK2A; | |
| AT5G07620 | 1.25 | 4.26 | 2 | Protein kinase family protein | |
| AT4G20790 | 1.25 | 0.52 | −1.36 | LRR family protein | |
| AT1G07650 | 11.63 | 27.88 | 1.17 | LRR transmembrane protein kinase | |
| AT1G53430 | 6.6 | 15.24 | 1.1 | LRR family protein/protein kinase family protein | |
| AT1G56120 | 0.63 | 1.39 | 1.02 | Receptor kinases LRR VIII.VIII-2 | |
| AT1G34420 | 0.68 | 1.47 | 1.31 | LRR family protein/protein kinase family protein | |
| AT1G09970 | 20.98 | 52.69 | 1.24 | LRR XI-23 | LRR XI-23 | |
| AT4G28490 | 2.84 | 6.41 | 1.08 | RLK5 HAE (HAESA) | |
| AT5G25930 | 1 | 3.04 | 1.52 | LRR family protein/protein kinase family protein | |
| AT1G35710 | 3.38 | 7.4 | 1.36 | LRR transmembrane protein kinase, putative | |
| AT3G47570 | 0.81 | 1.75 | 1.02 | LRR transmembrane protein kinase, putative | |
| AT4G08850 | 8.47 | 18.22 | 1.19 | Receptor kinases LRR XII | |
| AT4G39270 | 4.35 | 6.57 | 1 | LRR transmembrane protein kinase, putative | |
| AT2G23200 | 4.48 | 10.12 | 1.28 | Protein kinase family protein | |
| AT5G54380 | 11.16 | 23.85 | 1.02 | THE1 (THESEUS1); protein kinase | |
| AT4G21410 | 3.73 | 8.33 | 1.05 | Protein kinase family protein | |
| AT4G23130 | 1.11 | 2.56 | 1.12 | CRK5 (Cys-rich RLK5) | |
| AT4G23140 | 0.76 | 2.07 | 1.34 | CRK6 (Cys-rich RLK6) | |
| AT4G23230 | 0.8 | 2.26 | 1.4 | Protein kinase family protein | |
| AT1G66880 | 3.39 | 7.47 | 1.15 | Ser/Thr protein kinase family protein | |
| AT5G38240 | 1 | 2.96 | 1.45 | Ser/Thr protein kinase, putative | |
| AT2G18470 | 3.56 | 0.48 | −3.02 | Protein kinase family protein | |
| AT4G34440 | 1.32 | 0.49 | −1.54 | Protein kinase family protein | |
| AT3G12000 | 9.18 | 4.25 | −1.18 | S-locus-related protein SLR1, putative (S1) | |
| AT1G21250 | 7.22 | 21.76 | 1.49 | WAK1 (Cell wall-associated kinase 1) | |
| AT1G21270 | 2.41 | 5.28 | 1.03 | WAK2 (Cell wall-associated kinase 2) | |
| Small GTPases | RIC1 | 1.48 | 0.23 | −2.61 | Rop-Interactive Crib motif-containing protein 1 |
| RIC3 | 4.92 | 0.54 | −3.3 | Rop-Interactive Crib motif-containing protein 3 | |
| ROP1 | 7.91 | 3.02 | −1.48 | Rho-related protein from plants 1; GTP binding | |
| ROP7 | 3.89 | 1.48 | −1.44 | Arabidopsis RAC-like 2; GTP binding | |
| AT1G08340 | 8.18 | 2.9 | −1.58 | Rac GTPase activating protein, putative | |
| RABA2B | 9.76 | 4.7 | −1.13 | Arabidopsis rab GTPase homolog a2b | |
| RABA4D | 2.69 | 0.74 | −1.99 | RAB GTPase homolog A4d, GTP binding | |
| RABH1e | 7.55 | 2.85 | −1.47 | Arabidopsis Rab GTPase homolog H1e, GTP binding | |
| RABA6a | 4.8 | 2.38 | −1.11 | Arabidopsis Rab GTPase homolog A6a, GTP binding | |
| ROPGEF9 | 1.1 | 0.36 | −1.66 | ROPGEF9, Rho guanyl-nucleotide exchange factor | |
| PRA1.B5 | 2.85 | 1.32 | −1.57 | PRA1.B5 (Prenylated Rab Acceptor 1.b5) | |
| AT2G37290 | 1.84 | 0.94 | −1.05 | Rab GAP/TBC domain-containing protein | |
| AT5G09550 | 36.17 | 7.65 | −2.33 | RAB GDP-dissociation inhibitor | |
| AT1G12070 | 3.04 | 0.34 | −3.28 | Rho GDP-dissociation inhibitor family protein | |
| AT1G62450 | 10.99 | 1.72 | −2.78 | Rho GDP-dissociation inhibitor family protein | |
| Sugar Transporters and Water Channel | |||||
| Sugar transporters | SWEET3 | 33.58 | 17.98 | −0.98 | HXT |
| SWEET4 | 15.96 | 9.34 | −0.88 | HXT | |
| SWEET5 | 9.96 | 0.99 | −3.41 | HXT | |
| SWEET7 | 26.03 | 10.71 | −1.38 | HXT | |
| SWEET8 | 132.86 | 57.57 | −1.3 | HXT | |
| STP2 | 76.32 | 33.43 | −1.28 | STP2, Glc:hydrogen symporter | |
| STP6 | 17.54 | 1.79 | −3.35 | STP6, Glc:hydrogen symporter | |
| STP9 | 68.39 | 7.04 | −3.4 | STP9, Glc:hydrogen symporter | |
| TMT3 | 10.95 | 2.87 | −2.14 | TMT3 (tonoplast monosaccharide transporter3) | |
| TIP1-3 | 18.01 | 2.2 | −3.13 | TIP1;3 (tonoplast intrinsic protein 1;3) | |
| TIP5-1 | 10.88 | 2.64 | −2.13 | TIP1;5 (tonoplast intrinsic protein 1;5) | |
| PIP1-5 | 77.28 | 39.66 | −1.06 | PIP1;5 (plasma membrane intrinsic protein 1;5) | |
| PIP2-4 | 26.34 | 12.76 | −1.14 | PIP2;4 (plasma membrane intrinsic protein 2;4) | |
| PIP2B | 31.94 | 14.62 | −1.21 | PIP2B (plasma membrane intrinsic protein 2b) | |
| PIP2-3 | 31.16 | 13.01 | −1.36 | PIP2;3 (plasma membrane intrinsic protein 2;3) | |
| H+-ATPases | AHA6 | 49.51 | 9.1 | −2.53 | AHA6 (Arabidopsis H+-ATPase 6) |
| AHA9 | 36.46 | 8.08 | −2.28 | AHA9 (Arabidopsis H+-ATPase 9) | |