Transgenic expression of Gl2 and Gl2-like in Arabidopsis. A, Western blot analysis of the 46-kD GL2 protein transgenically expressed in either wild-type (WT) or cer2-5 mutant plants. Protein extract sample from maize silks serves as the positive control; protein extracts from the stems of wild-type Arabidopsis and cer2-5 mutant plants serve as negative controls. B, RT-PCR analysis of the Gl2-like mRNA heterologously expressed in either wild-type or cer2-5 mutant plants. Ubiquitin mRNA (At4g05320) is used as the internal control. C, Stem phenotypes of nontransgenic Arabidopsis wild-type and cer2-5 mutant, and transgenic lines expressing either maize Gl2 or CER2 in wild-type and cer2-5 mutant backgrounds.. D, Stem phenotypes of nontransgenic Arabidopsis wild-type and cer2-5 mutant, and transgenic lines expressing the maize Gl2-like transgene in either wild-type or cer2-5 mutant backgrounds.