Figure 1.
Expression pattern of PGLP2-1:GUS in transgenic rice plants at different growth stages. A, Specific expression in minor veins of the leaf in the vegetative phase. Leaves from 14-d-old seedlings: left, negative control (empty vector transformant [EV]) lacking signal; right, PGLP2-1:GUS leaf with strong blue GUS signal in minor veins. Leaves from 64-d-old plant: left, negative control; center, PGLP2-1:GUS leaf showing staining in the minor veins; right, CaMV 35S promoter (P35S) driving constitutive expression of GUS in the leaf. B, GUS staining in organs from mature plant, including leaf, spikelet, stem, internode, leaf sheath, and seed. Among these organs of PGLP2-1:GUS plants, light signal was observed in the leaf sheath and strong blue signal displayed specifically in the scutellum. C, GUS staining of developing seeds of PGLP2-1:GUS transformant. Seeds were sampled at different growth stages (17 and 35 DAF). Scutellum-specific expression pattern was exhibited by all developing seeds. EV seed was used as negative control, and P35S showing constitutive GUS expression was used as positive control.