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. 2020 Jun 26;10:916. doi: 10.3389/fonc.2020.00916

Figure 7.

Figure 7

Effect of CORO2A on cell migration in TNBC cell lines. (A) After transfection with CORO2A siRNA for 72 h, the mRNA expression level of CORO2A was assessed by RT-qPCR in MDA-MB-231 and BT549 cells. (B) After transfection with CORO2A siRNA or plasmid for 72 h, the protein expression level of CORO2A was assessed by Western blotting in MDA-MB-231 and BT549 cells. Lanes in Western blotting are denoted as: lane 1—NC, lane 2—si-CORO2A-1, lane 3—si-CORO2A-2, lane 4—pcDNA-3.1, and lanes 5—pcDNA-3.1-CORO2A. (C) Representative images of cell migration assays and the mean ± SEM (n = 3) of three independent experiments of quantitative cell migration assays in MDA-MB-231 and BT549 cells are shown following CORO2A knockdown. Cell migration measured by scratch-wound (D) and Transwell asssay (E) are shown following CORO2A overexpression in MDA-MB-231 and BT549 cells. *P < 0.05, and **P < 0.01 compared to corresponding negative controls (NC) or pcDNA-3.1.