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. 2020 Jun 26;11:1149. doi: 10.3389/fimmu.2020.01149

Figure 4.

Figure 4

S1P induced IL-1β and IL-18 secretion in NLRP3 inflammasome-dependent manner. (A) Representative images of immunofluorescence analysis by confocal microscopy in serum-starved BMMs treated with 1 μmol/L S1P for 6 h. White arrows indicate oligomerized ASC. (B) The relative expression of NLR and non-NLR PRR family members were examined by qRT-PCR in serum-starved BMMs treated with S1P for 2 h. (C) ELISA measurements of IL-1β and IL-18 in culture supernatants from serum-starved BMMs pretreated with 100 nmol/L MCC950 for 1 h, followed by treatment with 1 μmol/L S1P for 12 h, or 100 ng/ml LPS for 4 h, then 5 mmol/L ATP for 30 min. Data are presented as the mean ± SEM. n = 6 per group. Student's t-test was used in panel (B) and one-way ANOVA was used in panel (C). *p < 0.05 vs. control. #p < 0.05 vs. S1P treatment alone. &p < 0.05 vs. LPS plus ATP treatment.