Table 1.
Primer sequence.
| Mouse | Sequence | |
|---|---|---|
| 18s rRNA | Sense | GTAACCCGTTGAACCCCATT |
| Antisense | CCATCCAATCGGTAGTAGCG | |
| sphingosine kinase 1 (Sphk1) | Sense | ATGGAACCAGTAGAATGCCCT |
| Antisense | TCCGTTCGGTGAGTATCAGTTTA | |
| NLR family, pyrin domain containing 3 (Nlrp3) | Sense | ATTACCCGCCCGAGAAAGG |
| Antisense | TCGCAGCAAAGATCCACACAG | |
| interleukin 1 beta (Il1β) | Sense | GCAACTGTTCCTGAACTCAACT |
| Antisense | ATCTTTTGGGGTCCGTCAACT | |
| interleukin 18 (Il18) | Sense | GACTCTTGCGTCAACTTCAAGG |
| Antisense | CAGGCTGTCTTTTGTCAACGA | |
| sphingosine-1-phosphate receptor 1 (S1pr1) | Sense | ACTTTGCGAGTGAGCTG |
| Antisense | AGTGAGCCTTCAGTTACAGC | |
| sphingosine-1-phosphate receptor 2 (S1pr2) | Sense | TTCTGGAGGGTAACACAGTGGT |
| Antisense | ACACCCTTTGTATCAAGTGGCA | |
| sphingosine-1-phosphate receptor 3 (S1pr3) | Sense | TGGTGTGCGGCTGTCTAGTCAA |
| Antisense | CACAGCAAGCAGACCTCCAGA | |
| NLR family, CARD domain containing 4 (Nlrc4) | Sense | ATCGTCATCACCGTGTGGAG |
| Antisense | GCCAGACTCGCCTTCAATCA | |
| absent in melanoma 2 (Aim2) | Sense | GTCACCAGTTCCTCAGTTGTG |
| Antisense | CACCTCCATTGTCCCTGTTTTAT | |
| NLR family, pyrin domain containing 1B (Nlrp1b) | Sense | AGTAATCTGGAGGGGTTGGAC |
| Antisense | GTTGGCAGCCAGGGTATATCA | |
| NLR family, CARD domain containing 3 (Nlrc3) | Sense | CAGATTGGTAACAAAGGAGCCA |
| Antisense | CGTTCGGTTTATCTTCAGAGCA | |
| NLR family, pyrin domain containing 2 (Nlrp2) | Sense | GAAAGCTGGACAAGACTGAGTT |
| Antisense | GGCAGTGGTCTGTGAGAATTT | |
| NLR family, pyrin domain containing 4a (Nlrp4a) | Sense | GGATGCCCAAAGTTATATCGAGC |
| Antisense | CCAGGCCAGCATTAACCTCTT | |
| NLR family, pyrin domain containing 5 (Nlrp5) | Sense | GAAAGCACAATGGGTCCTCCA |
| Antisense | CTGACGCCTGTTCCACTTCT | |
| NLR family, pyrin domain containing 6 (Nlrp6) | Sense | CTCGCTTGCTAGTGACTACAC |
| Antisense | AGTGCAAACAGCGTCTCGTT | |
| NLR family, pyrin domain containing 9a (Nlrp9a) | Sense | CTTTGCTGCAATATCCAAGGGA |
| Antisense | GGACAGCGGCATAAATTGAACA | |
| NLR family, pyrin domain containing 10 (Nlrp10) | Sense | TCAAGACGCTGAAGTTCCACT |
| Antisense | TGCTCCGTACATTGAAATCAGTT | |
| NLR family, pyrin domain containing 12 (Nlrp12) | Sense | AAGACCGCAATGCACGATTAG |
| Antisense | TGGAGCGTTCCCACTCTACA | |
| NLR family, pyrin domain containing 14 (Nlrp14) | Sense | TCCACAAACGGTAGTCCTTCA |
| Antisense | ACTTGTCCCTGTTCAATGGGG | |
| NLR family member X1 (Nlrx1) | Sense | TAGGGCCTTTATCCGTTACCA |
| Antisense | TAAACCACTCGGTGAGGTTCC | |
| NLR family, apoptosis inhibitory protein 1 (Naip1) | Sense | TGCCCAGTATATCCAAGGCTAT |
| Antisense | AGACGCTGTCGTTGCAGTAAG | |
| NLR family, apoptosis inhibitory protein 2 (Naip2) | Sense | AGCTTGGTGTCTGTTCTCTGT |
| Antisense | GCGGAAAGTAGCTTTGGTGTAG | |
| NLR family, apoptosis inhibitory protein 5 (Naip5) | Sense | TGCCAAACCTACAAGAGCTGA |
| Antisense | CAAGCGTTTAGACTGGGGATG | |
| NLR family, apoptosis inhibitory protein 6 (Naip6) | Sense | TACAGGGAGTTTACAAGACCCC |
| Antisense | AGTGGCCTGGAGAGACTCAG | |
| Human | Sequence | |
| NLR family, pyrin domain containing 3 (NLRP3) | Sense | GATCTTCGCTGCGATCAACAG |
| Antisense | CGTGCATTATCTGAACCCCAC | |
| interleukin 1 beta (IL1β) | Sense | TTCGACACATGGGATAACGAGG |
| Antisense | TTTTTGCTGTGAGTCCCGGAG | |
| interleukin 18 (IL18) | Sense | TCTTCATTGACCAAGGAAATCGG |
| Antisense | TCCGGGGTGCATTATCTCTAC | |
| sphingosine kinase 1 (SPHK1) | Sense | AGAGTGGGTTCCAAGACACCT |
| Antisense | GGGTGCAGCAAACATCTCAC | |