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. 2020 Jun 29;13:6171–6180. doi: 10.2147/OTT.S256238

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© 2020 Sun et al.

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circ_0005394 promotes HCC cell progression. (A) circ_0005394 expression was detected after silencing of circ_0005394 in Huh-7 cells by qRT-PCR. (B) circ_0005394 expression was detected after overexpression of circ_0005394 in HepG2 cells by qRT-PCR. (C, D) CCK-8 assays were used to detect cell viability of Huh-7 and HepG2 cells after circ_0005394 knockdown/overexpression. (E, F) AO/EB staining assays were used to detect cell apoptosis of Huh-7 and HepG2 cells after circ_0005394 knockdown/overexpression. (G, H) Flow cytometric analysis was used to detect cell apoptosis of Huh-7 and HepG2 cells after circ_0005394 knockdown/overexpression. (I, J) Wound healing assays were used to detect cell migration capacities of Huh-7 and HepG2 cells after circ_0005394 knockdown/overexpression. (K, L) Transwell assays were used to detect cell migration and invasion capacities of Huh-7 and HepG2 cells after circ_0005394 knockdown/overexpression. * P<0.05, ** P<0.01.