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. 2020 Jun 29;13:6171–6180. doi: 10.2147/OTT.S256238

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© 2020 Sun et al.

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circ_0005394 sponges miR-507 and miR-515-5p to upregulate E2F3 and CXCL6 expression, respectively. (A) Relative miRNAs expression was detected after circ_0005394 knockdown in Huh-7 cells. (B) Relative miRNAs expression was detected after circ_0005394 overexpression in HepG2 cells. (C, D) Correlation analysis of circ_0005394 and miR-507/miR-515-5p in HCC patients’ tissues. (E) Diagrammatic sketch of the binding sites for circ_0005394 and miR-507/miR-515-5p. (F, G) Luciferase reporter assay was conducted to evaluate the interaction ability between miR-507/miR-515-5p and circ_0005394. (H) Relative E2F3 mRNA expression was measured after miR-507 knockdown/overexpression in Huh-7 and HepG2 cells. (I) Relative CXCL6 mRNA expression was measured after miR-515-5p knockdown/overexpression in Huh-7 and HepG2 cells. (J, K) Correlation analysis of circ_0005394 and E2F3/CXCL6 mRNA in HCC patients’ tissues. (L) Diagrammatic sketch of the binding sites for E2F3 3ʹ-UTR/miR-507 and CXCL6 3ʹ-UTR/miR-515-5p. (M) Luciferase reporter assay was conducted to evaluate the interaction ability between miR-507 and E2F3 3ʹ-UTR. (N) Luciferase reporter assay was conducted to evaluate the interaction ability between miR-515-5p and CXCL6 3ʹ-UTR. * P<0.05, ** P<0.01.