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. 2020 May 28;9:e55761. doi: 10.7554/eLife.55761

Figure 2. ADP integral correlates with apical trunk length.

(A) Summary data of maximum ADP integral values for all recorded neurons. The dashed line denotes the division between the two groups of cells classified through k-means clustering; asterisk denotes p = 4.54 * 10−6, D = 0.52; two-sample Kolmogorov-Smirnov test. (B) Proportion of cells with cfADP in V1 and V2m. (C) Length of the apical trunk (soma to main bifurcation) plotted against the corresponding maximum ADP integral values. Dashed line is a linear fit (r2 = 0.154, p = 5.37*10-3, F-test); curves at the top and right are kernel density plots of the two variables in V1 and V2m; asterisk denotes p = 4.26*10−6, two-sample t-test.

Figure 2.

Figure 2—figure supplement 1. Maximum ADP integral for all cells split by recording ACSF containing either 1.5 or 2 mM CaCl2.

Figure 2—figure supplement 1.

p and D values are shown for a two-sample Kolmogorov-Smirnov test. All cells recorded during this study in 1.5 mM CaCl2 are included.
Figure 2—figure supplement 2. Maximum ADP integral for all cells including V1 (grey), retro-labelled cells recorded from V2m (blue), and V2m cells labelled in the Colgalt2-Cre mouse line (cyan).

Figure 2—figure supplement 2.

p and D value are shown for a two-sample Kolmogorov-Smirnov test. All cells recorded during this study from Colgalt2-Cre animals are included.
Figure 2—figure supplement 3. Intrinsic properties of in vitro and in silico recorded neurons.

Figure 2—figure supplement 3.

(A) Representative voltage traces for V1 and V2m neurons in response to 500 ms wide depolarizing current steps. The traces show stimulation at 60 pA (grey) and 180 pA (coloured or black) above rheobase for each cell. (B) Same as in A but for the detailed model with the long and short morphology. (C) Same as in A but for the reduced model with a trunk length of either 600 μm or 200 μm. (D) Quantification of a range of biophysical properties for both recorded and model neurons. Asterisk denotes p < 0.0083 (Bonferroni-corrected).
Figure 2—figure supplement 4. Reconstructions and morphological analysis of V1 and V2m neurons.

Figure 2—figure supplement 4.

(A) Full reconstructions of 6 neurons in V1 (top) and 7 neurons in V2m (bottom), labelled with CTB and filled with biocytin during recordings. Black: dendrites and soma. Green: axons. (B) Quantification of morphological parameters from the reconstructed neurons shown in A. Asterisk denotes p < 0.005 Bonferroni-corrected two-sample t-test. (C) Sholl analysis, showing number of dendritic intersections with concentric spheres in 10 μm increments from the soma.