TABLE III.
Gel recipes in μl for the total of 500 μl gel mix. The underlined numbers are for gels made with the NHS in the DMSO method where 50 μl of NHS in 4% DMSO is added to the gel mix.
| Gel G′(k Pa), G″(Pa) | Acrylamide 40% stock | Bis-acrylamide 2% stock | H2O | TEMED | APS | Linear PAA |
|---|---|---|---|---|---|---|
| 1 kPa, 1 Pa | 56 | 25 | 414 | 1.25 | 3.75 | … |
| 56 | 25 | 364 | 1.25 | 3.75 | … | |
| 1 kPa, 200 Pa | 69 | 25 | 80 | 1.25 | 3.75 | 321 |
| 69 | 25 | 30 | 1.25 | 3.75 | 321 | |
| 5 kPa, 10 Pa | 100 | 25 | 370 | 1.25 | 3.75 | … |
| 100 | 25 | 320 | 1.25 | 3.75 | … | |
| 5 kPa, 500 Pa | 100 | 37.5 | 336 | 1.25 | 3.75 | 286 |
| 100 | 37.5 | 286 | 1.25 | 3.75 | 286 |