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. 2020 Jul 3;3:344. doi: 10.1038/s42003-020-1071-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, d Purified G478del (a) and G460del (d) proteins analyzed by SDS-PAGE. b, e The EMSAs for G478del (b) and G460del (e). Various amounts of G478del (b) or G460del (e) (ranging from 0 to 13 μM) were mixed with 1 μM of 10L10_UGU or 10L10_noUGU in a 10 μL reaction solution. c, f Quantification of the EMSA data in (b) and (e) for G478del and G460del, respectively. The density of each RNA band on the gel was measured using Image Lab 6.0 (Bio-Rad), and the results were obtained from three independent experiments.