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. 2020 Jul 3;3:344. doi: 10.1038/s42003-020-1071-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a The DGCR8ΔCTT knockout (KO) cells were transfected with pCK (control), pCK-DGCR8–WT, or pCK-DGCR8–mut1. The miRNA expression of these transfected cells was estimated by qPCR as described in “Methods”. The results were obtained from three independent experiments (miR-16-5p DGCR8/control vs. miR-16-5p DGCR8–mut1/control: p = 0.857, miR-30a-5p DGCR8/control vs. miR-30a-5p DGCR8–mut1/control: p = 0.010). b The pri-miRNA expression in the transfected cells in (a) was estimated by qPCR and normalized against GAPDH. The results were obtained from three independent experiments (pri-mir-16-1 DGCR8/control vs. pri-mir-16-1 DGCR8–mut1/control: p = 0.904, pri-mir-30a DGCR8/control vs. pri-mir-30a DGCR8–mut1/control: p = 0.651). c The miR-30a expression in the DGCR8ΔCTT KO cells transfected with pCK-DGCR8–WT or mut1, pcDNA3-pri-mir-16-1, and pcDNA3-pri-mir-30a_UGU or pcDNA3-pri-mir-30a_noUGU. The results were obtained from three independent experiments (for the pcDNA3-pri-mir-30a_UGU transfected cells: miR-16-5p DGCR8–WT/control vs. miR-16-5p DGCR8–mut1/control: p = 0.322, miR-30a-5p DGCR8–WT/control vs. miR-30a-5p DGCR8–mut1/control: p = 5.5e−5; for the pcDNA3-pri-mir-30a_noUGU transfected cells: miR-16-5p DGCR8–WT/control vs. miR-16-5p DGCR8–mut1/control: p = 0.161, miR-30a-5p DGCR8–WT/control vs. miR-30a-5p DGCR8–mut1/control: p = 2.2e−4). d The expression of pri-miRNAs in the transfected cells described in (c). The pri-miRNA expression was estimated by qPCR and normalized using GAPDH. The results were obtained from three independent experiments (for the pcDNA3-pri-mir-30a_UGU transfected cells: pri-mir-16-1 DGCR8–WT/control vs. pri-mir-16-1 DGCR8–mut1/control: p = 0.792, pri-mir-30a DGCR8–WT/control vs. pri-mir-30a DGCR8–mut1/control: p = 0.968; for the pcDNA3-pri-mir-30a_noUGU transfected cells: pri-mir-16-1 DGCR8–WT/control vs. pri-mir-16-1 DGCR8–mut1/control: p = 0.364, pri-mir-30a DGCR8/control vs. pri-mir-30a DGCR8–mut1/control: p = 0.897. The asterisks (*) and (ns) indicate statistically significant and nonsignificant differences, respectively, from the two-sided t test. e The cumulative fraction of miRNA expression levels in the rescued DGCR8ΔCTT KO cells. The miRNAs were classified according to the presence of an apical UGU motif in pri-miRNAs as described in “Methods”. Cohenʼs d values were calculated between the miRNA expression levels induced by DGCR8–WT and DGCR8–mut1. The p values were calculated with the two-sided Wilcoxon rank-sum test.