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. 2020 Jul 3;3:351. doi: 10.1038/s42003-020-1081-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a RNA sequencing showed that 60 yir genes are expressed (count >20) and are divided into three groups based on the yir expression relative to that of the wild-type YM parasites. The blue arrow points to the gene that was tagged in this transfected cell line. Orange arrows point to the genes selected for qRT-PCR validation. b Expression of yirs in each group were validated using qRT-PCR and results are in line with RNA sequencing results. All data are presented as mean ± standard error of the mean (SEM).