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. 2020 Jul 3;3:341. doi: 10.1038/s42003-020-1067-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative immunofluorescence images of H3K4me1/2/3, H3K36me2, and H3K9me3 for B16F0 cells cultured in a panel of shapes. b Flow cytometry characterization of histone methylation in B16F0 cells and quantification of the difference between patterned and non-patterned cells for each condition with thresholds (green dots) assigned by overlapping regions of patterned and non-patterned plots. c Single cell analysis of histone methylation in B16F0 cells cultured in perimeter or central regions of the circular geometry (n = 3, biological replicates, total 100 cells for each marker). Boxes represent 25^th to 75^th percentile and whiskers represent minimum-maximum. Horizontal lines and points within boxes represent the median and mean respectively for three duplicates. Scale bars, 50 μm. *P < 0.05, ANOVA. Error bars represent s.d.