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. 2020 Jul 3;10:10976. doi: 10.1038/s41598-020-67345-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression of COX pathway. The microarray and qRT-PCR were performed with 2 × 10⁵ human neutrophils from 3 different donors, stimulated with Cr-LAAO (50 μg/mL), LPS (1 μg/mL; positive control) or RPMI (negative control) for 1 h at 37 °C and 5% CO₂. This figure was created using images from Servier Medical Art Commons Attribution 3.0 Unported License (https://smart.servier.com) (A). Servier Medical Art by Servier is licensed under a Creative Commons Attribution 3.0 Unported License. The microarray fold change was represented in up regulation (red) and down regulation (green) for cells stimulated with Cr-LAAO in relation to control. The genes shown were for phospholipase A2-activating protein (PLAA), group IVA (PLA2G4A), IVB (PLA2G4B), IVC (PLA2G4C), IVD (PLA2G4D), IVE (PLA2G4E), IVF (PLA2G4F) and VI (PLA2G6) phospholipase A2, prostaglandin-endoperoxide synthase 1 (PTGS1) and 2 (PTGS2), prostaglandin E synthase 1 (PTGES), 2 (PTGES2) and 3 (PTGES3), prostaglandin reductase 1 (PTGR1) and 2 (PTGR2), prostaglandin E receptor 1 (PTGER1), 2 (PTGER2), 3 (PTGER3) and 4 (PTGER4), perilipin 1–5 (PLIN1, PLIN12, PLIN3, PLIN4 and PLIN5), diacylglycerol O-acyltransferase 1 and 2 (DGAT1 and DGAT2) (B). The genes analyzed by qRT-PCR were PLAA, PLA2G4A, PTGS1, PTGS2, PTGES and PLIN2. Hemoglobin subunit beta (HBB) was used as reference gene for normalization (C). Values are mean S.E.M. from 3 donors. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to negative control (Data were presented with ANOVA followed by Dunnett post-test).