Figure 4.

Production of lipid bodies in neutrophils pretreated with DGAT and cPLA₂ inhibitor. LBs quantification stained with neutrophils (2 × 10⁵) pre-treated or not with inhibitors CAY10650 (cPLA₂) and A922500 (DGAT) and stimulated Cr-LAAO (50 μg/mL), LPS (1 μg/mL; positive control) or RPMI (negative control) for 1 h at 37 °C and 5% CO₂. This figure was created using images from Servier Medical Art Commons Attribution 3.0 Unported License (https://smart.servier.com) (A). Servier Medical Art by Servier is licensed under a Creative Commons Attribution 3.0 Unported License. Figure representative of one experiment of three independent experiments (B). The mean number of LBs from neutrophils stimulated in the absence of fetal bovine serum (FBS) (C) and in the presence of fetal bovine serum (D) was measured in 50 cells in the vision field of each condition impartially collected from 3 donors. Values are mean S.E.M. from 3 donors. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to negative control, and ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 compared to without pre-treatment with inhibitor (Data were presented with ANOVA followed by Tukey post-test).