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. 2020 Jul 3;3:338. doi: 10.1038/s42003-020-1028-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b Pitx2 mRNA primarily detected in the STN but also seen in the PSTN, LH, PH, ZI, MM and RM. c–r FISH analysis of Pitx2 mRNA (green) combined with 16 selected markers (red) within the STN, PSTN, ZI, LH and PH (in order presented: Htr2c; Nxph1; Nxph4; Kcnab3; Fgf11; Stxbp2; Glra3; Pvalb; Col24a1; Nmbr; Calb2; Adcyap1; Baiap3; Cacna2d3; Tac1; Synpr mRNAs). Regional distribution detected for all mRNAs in the ventral diencephalic areas assessed. All mRNAs found in the STN except Baiap3, Cacna2d3, Glra3, Tac1, Synpr. All mRNAs in the STN co-localize with Pitx2. Co-localization of mRNA with Pitx2 mRNA is shown in yellow. Scale bar 250 µm. Abbreviations: LH, Lateral hypothalamus; MM, Medial mammillary nucleus; PSTN Parasubthalamic nucleus; PH, Posterior hypothalamus, RM, Retromammillary nucleus; STN: Subthalamic nucleus; ZI, Zona incerta.