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. 2020 Jul 3;3:338. doi: 10.1038/s42003-020-1028-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a–r Combinatorial FISH analysis comparing mRNAs towards each other allows distinction into STNa/STNb/STNc/STNds domains based on detection of several mRNAs. Three levels along the antero-posterior axis analyzed for complete revelation across the STN structure. mRNAs analyzed, in order presented: a Pitx2/Vglut2; b Pitx2/Calb2; c Pitx2/Baiap3; d Htr2c/Baiap3; e Kcnab3/Baiap3; f Col24a1/Kcnab3; g Pitx2/Glra3; h Pitx2/Tac1; i Htr2c/Tac1; j Pitx2/Gad1; k Pitx2/Htr2c; l Htr2c/Nxph1; m Htr2c/Nxph4; n Nxph1/Nxph4; o Pitx2/Col24a1; p Pvalb/Pitx2; q Pvalb/Col24a1; r Tac1/Calb2 presented in green/red with corresponding color of arrows and with white arrow heads representing co-localization. Scale bar 200 µm and 125 µm (insets). Abbreviations: dsSTN, dorsal strip of STN; FISH, fluorescent in situ hybridization; STN, Subthalamic nucleus; PSTN, Parasubthalamic nucleus; ZI, Zona incerta.