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. 2020 Jun 26;14(2):243–264. doi: 10.3897/CompCytogen.v14i2.51154

Table 2.

The distribution of fluorochrome bands and rDNA sites in the five wild Vigna species.

Species Fluorochrome bands Number (pairs) and location of rDNA sites
Type Distribution Amount (%) Band size (mean)§ 5S| 45S|
V. luteola CPD all CENs, PCENs and 45S sites 29.19 1.98–3.21 (2.65) one [11S-PCEN (16.55%)] one (3S)
V. vexillata CPD all 45S sites one [(8L-INT(52.29%)] two [2S-TER (20.53%), 3S-TER (16.73%)]
V. minima CPD all 45S and 5S sites one [2S-INT (30.86%)] five [2L-TER (58.64%), 4L-TER (59.42%), 6S-TER (38.91%), 7S-TER (50.74%), 9S-TER (67.94%)]
V. trilobata CPD all CENs, PCENs and 45S sites 20.04 2.73–1.12 (1.82) one [4L-PCEN(14.95%)] two [6S-PCEN(25.05%), 7S]
V. caracalla CPD all CENs, PCENs and 45S sites, 4S-, 4L-, 5L-INTs 21.68 0.89–2.63 (1.55) two [2L-INT(34.32%), 5S-INT (56.7%)] one (1S)
DAPI 2, 3, 4, 5S-PCENs; 4, 5, 6, 8L-PCENs 8.19 0.69–1.38 (1.04)

S and L represent short and long arms, respectively; CEN, PCEN, INT and TER represent centromeric, pericentromeric, interstitial, terminal position, respectively; figures ahead of the positions are the designations of the chromosome pair involved. Amount of bands in the genome expressed as percentage of the karyotype length (rDNACPD bands are excluded). §The percentage of the size of the bands of each chromosome pair in relation to the karyotype length. |The percentages in square brackets are the percentage distance from centromere to the rDNA site (di = d× 100/a; d = distance of starting point of terminal sites judged by CPD bands or center of non-terminal sites judged by the FISH signals from the centromere, a = length of the corresponding chromosome arm). The value consists of the amounts of centromeric and pericentromeric CPD bands (18.29%) as well as interstitial CPD bands (3.39%).