Table 2.
Method performance parameters for measurement of NCHs. Flowrate 300 μL/min, 5 μL injection volume. Separation column: Primesep 200, Gradient according to ESM Table S3. MS conditions according to ESM Table S4
| Compound | Retention (tR) [min] | Peak width (W50) [min] | Peak asymmetry (As) | Retention factor (k′) | Selectivity (α) | Efficiency (N) [plates/column] | Resolution (Rs) |
|---|---|---|---|---|---|---|---|
| Imidazole | 8.93 | 0.29 | 1.03 | 3.65 | 1.74 | 4399 | 5.55 |
| Pyrazole | 5.94 | 0.26 | 1.65 | 2.09 | 1.18 | 2960 | 1.43 |
| Pyridine | 12.48 | 0.22 | 1.27 | 5.50 | 1.51 | 17424 | 9.40 |
| Pyridazine | 5.31 | 0.24 | 1.32 | 1.77 | – | 2410 | – |
| Piperidine | 12.92 | 0.23 | 1.16 | 5.73 | 1.04 | 17062 | 1.12 |