Fig. 5.

Characterization of acid compartments on parasitophorous vacuoles. The figure shows images of J774 macrophages with amastigotes of L. pifanoi labeled with LysoTracker. A. Control, B Positive control cells were incubated with Nigericin 10 μM. C: TC1, D: TC2, E: SS, F: TC1:SS, G: TC2:SS. Red arrows indicated the polar localization of the acid compartment in the cells. The numbers in the image correspond to the AUF (arbitrary units of fluorescence) by analysis in ImageJ. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)